ER Lipid Raft Associated 2 (ERLIN2) antibody

Antigen: ER Lipid Raft Associated 2 (ERLIN2)

Synonyms: NET32, SPFH2, C8orf2, Erlin-2, MGC87072, MGC137889

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185458

Additional Information

Alternative name: SPFH2 / ERLIN2

Gene ID: 11160, 290823 (rat)

Immunogen: Peptide with sequence C-EDEPLETATKEN, from the C Terminus of the protein sequence according to NP_009106.1. Please note the peptide is available for sale.

Format: Affinity-purified

Description: Goat Anti-SPFH2 / ERLIN2 Antibody. All names and symbols: ERLIN2, SPFH2, ER lipid raft associated 2, SPFH domain family, member 2 , HGNC:1356, C8orf2, MGC87072 , chromosome 8 open reading frame 2. Official Symbol: ERLIN2. RefSeq number(s): NP_009106.1

Specificity: This antibody is expected to recognise isoform 1 (NP_009106) only. Tested: Human. Expected from sequence similarity: Human, Rat.

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376). Recommended concentration: 0.3-1µg/ml.

Concentration: 0.5 mg/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185458 staining (1µg/ml) of Human Breast lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Garcia, Pole, Chin et al.: "A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes." in: Oncogene, Vol. 24, Issue 33, pp. 5235-45, 2005 (PubMed).