Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) antibody

Antigen: Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1)

Synonyms: M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, Pca, ttw, twy, Npps, Ly-41, Pca-1, Pdnp1, C76301, CD203c, E-NPP1, AI428932, 4833416E15Rik, Pc1, ENPP1, MGC136238, zgc:136238, si:ch211-142e24.2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185487

Additional Information

Alternative name: ENPP1 / PC1

Gene ID: 5167

Immunogen: C-KTHLPTFSQED

Description: All names and symbols: ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1 , Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membrane glycoprotein 1. Official Symbol: ENPP1. RefSeq number(s): NP_006199.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 150-170kDa band in Human Liver and Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 99.9kDa according to NP_006199.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on Human Pancreas showing membranous staining of acinar cells. Recommended concentration, 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185487 (1µg/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Publications

Meyre, Bouatia-Naji, Tounian et al.: "Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes." in: Nature genetics, Vol. 37, Issue 8, pp. 863-7, 2005 (PubMed).