Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 Regulator 2 (SLC9A3R2) antibody

Antigen: Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 Regulator 2 (SLC9A3R2)

Synonyms: SIP1, OCTS2, SIP-1, TKA-1, E3KARP, NHERF2, NHE3RF2, NHERF-2, MGC104639, cb895, zgc:55541, zgc:77629, wu:fd20a02, SLC9A3R2, MGC133988

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185496

Additional Information

Alternative name: SLC9A3R2 (isoform A3)

Gene ID: 9351

Immunogen: C-DGSAWKQDPFQ

Format: Affinity-purified

Description: All names and symbols: SLC9A3R2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2, HGNC:11076, E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1 , sodium/hydrogen exchanger, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2, solute carrier family 9 isoform 3 regulator 2. Official Symbol: SLC9A3R2. RefSeq number(s): BAA33216.1

Specificity: Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 38kDa band observed in human kidney lysates (calculated MW of 37.4kDa according to BAA33216.1). Recommended concentration: 0.3-1µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows strong membranous staining in glomerulus-. Recommended concentration, 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185496 (0.3µg/ml) staining of human kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185496 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Cha, Kim, Hut et al.: "cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 16642-50, 2005 (PubMed).