Stratifin (SFN) antibody

Antigen: Stratifin (SFN)

Synonyms: YWHAS, Er, Mme1, Ywhas, LOC408037, LOC100220844

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185539

Additional Information

Alternative name: 14-3-3 sigma / Stratifin

Gene ID: 2810

Immunogen: C-DLHTLSEDSYKDST

Format: Affinity-purified

Description: All names and symbols: 14-3-3sigma, stratifin, HGNC:10773 , SFN. Official Symbol: SFN. RefSeq number(s): NP_006133.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer . Recommended concentration, 2-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185539 (0.1µg/ml) staining of human skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185539 (2.5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Lodygin, Hermeking: "The role of epigenetic inactivation of 14-3-3sigma in human cancer." in: Cell research, Vol. 15, Issue 4, pp. 237-46, 2005 (PubMed).