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Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) antibody

Antigen

Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA)

Synonyms CYCA, MGC72881, PPIA, Cyp1, PPIA-2, fa93g09, wu:fa93g09, wu:fb05e11, wu:fb13h02, wu:fb15a05, MGC75715, CypA, LOC574102, Cypa
Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN185542
Quantity 100 ug
Price 374.27 $   Plus shipping costs $45.00
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Additional Information

Alternative name Cyclophilin A / PPIA
Gene ID 5478
Immunogen C-ERFGSRNGKTSKK
Description All names and symbols: PPIA, peptidylprolyl isomerase A (cyclophilin A) , HGNC:9253, CYPA, CYPH, MGC12404 , T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase. Official Symbol: PPIA. RefSeq number(s): NP_066953.1, NP_982254.1, NP_982255.1
Specificity This antibody is expected to recognise both isoform 1 (NP_066953.1) and isoform 2 (NP_982254.1 and NP_982255.1). This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to isoform 1 (NP_066953.1). Human, Mouse, Rat, Dog

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 16kDa band observed in human lymph node, tonsil and thymus lysates (calculated MW of 18.0kDa according to NP_066953.1 and NP_001008741.1). Recommended concentration: 1-3µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Berthoux, Sebastian, Sokolskaja et al.: "Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 41, pp. 14849-53, 2005 (PubMed).

Alternatives

Alternatives for antigen "Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA)", type "Antibodies"
Hosts Rabbit (71), Goat (4), Mouse (4)
Reactivities Human (63), Mouse (Murine) (41), Rat (Rattus) (41), Cow (Bovine) (25), Pig (Porcine) (23), Dog (Canine) (22), Rabbit (4), Chicken (3), Rhesus Monkey (2), Wild boar (Sus scrofa) (2), Xenopus laevis (2), Zebrafish (1)
Applications Western Blotting (WB) (60), ELISA (36), Immunohistochemistry (IHC) (29), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), To be determined by user (TBD) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Biotin (8), FITC (4), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes Internal Region (4), N-Term (2), AA 2-165 (1), C-Term (1)