Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) antibody

Antigen: Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA)

Synonyms: CYCA, MGC72881, PPIA, Cyp1, PPIA-2, fa93g09, wu:fa93g09, wu:fb05e11, wu:fb13h02, wu:fb15a05, MGC75715, CypA, LOC574102, Cypa

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185542

Additional Information

Alternative name: Cyclophilin A / PPIA

Gene ID: 5478

Immunogen: C-ERFGSRNGKTSKK

Format: Affinity-purified

Description: All names and symbols: PPIA, peptidylprolyl isomerase A (cyclophilin A) , HGNC:9253, CYPA, CYPH, MGC12404 , T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase. Official Symbol: PPIA. RefSeq number(s): NP_066953.1, NP_982254.1, NP_982255.1

Specificity: This antibody is expected to recognise both isoform 1 (NP_066953.1) and isoform 2 (NP_982254.1 and NP_982255.1). This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to isoform 1 (NP_066953.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 16kDa band observed in human lymph node, tonsil and thymus lysates (calculated MW of 18.0kDa according to NP_066953.1 and NP_001008741.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185542 (2µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Berthoux, Sebastian, Sokolskaja et al.: "Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 41, pp. 14849-53, 2005 (PubMed).