Laminin, alpha 3 (LAMA3) antibody

Antigen: Laminin, alpha 3 (LAMA3)

Synonyms: E170, LOCS, BM600, LAMNA, lama3a, [a]3B, Lama3B, LAMA3

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN185545

Additional Information

Alternative name: LAMA3

Gene ID: 3909

Immunogen: NNQCHSSHKRRTK

Format: Affinity-purified

Description: All names and symbols: LAMA3, laminin, alpha 3 , HGNC:6483, E170, LAMNA, LOCS , BM600 150kD subunit, epiligrin 170 kda subunit, epiligrin alpha 3 subunit, kalinin 165kD subunit, laminin alpha 3 subunit, laminin alpha 3b chain, laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin), laminin-5 alpha 3 chain, nicein 150kD subunit. Official Symbol: LAMA3. RefSeq number(s): NP_937762.1

Specificity: This antibody is expected to recognise isoform 1 (NP_937762.1) only. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Preliminary experiments in human kidney, lung and skin lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Dietze, Bowie, Mrozek et al.: "CREB-binding protein regulates apoptosis and growth of HMECs grown in reconstituted ECM via laminin-5." in: Journal of cell science, Vol. 118, Issue Pt 21, pp. 5005-22, 2005 (PubMed).