rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5) antibody

Antigen: rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5)

Synonyms: ARHGEF5, MGC138953, P60, TIM, GEF5, TIM1, DKFZp686N1969

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185572

Additional Information

Alternative name: GEF5

Gene ID: 7984

Immunogen: C-DKEIDQNSQQEE

Format: Affinity-purified

Description: All names and symbols: GEF5, DKFZp686N1969, ARHGEF5, Rho guanine nucleotide exchange factor (GEF) 5, P60, TIM, TIM1, guanine nucleotide regulatory protein TIM, oncogene TIM rho guanine nucleotide exchange factor 5, transforming immortalized mammary oncogene. Official Symbol: ARHGEF5. RefSeq number(s): NP_005426.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185572 (0.1µg/ml) staining of HeLa Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Debily, Camarca, Ciullo et al.: "Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer." in: Human molecular genetics, Vol. 13, Issue 3, pp. 323-34, 2004 (PubMed).