Allograft Inflammatory Factor 1 (AIF1) (isoform 3) antibody

Antigen: Allograft Inflammatory Factor 1 (AIF1)

Synonyms: IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1

Epitope: isoform 3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185597

Additional Information

Alternative name: AIF1 / IBA1 (isoform 3)

Gene ID: 199, 11629 (mouse)

Immunogen: C-NKQFLDDPKYSSDED

Format: Affinity-purified

Description: All names and symbols: AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule. Official Symbol: AIF1. RefSeq number(s): NP_001614.3

Specificity: This antibody is expected to recognise isoform 3 (NP_001614.3) only. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 17kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 1-3µg/ml. Additional bands of unknown identity were also consistently observed at 35kDa and 110kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185597 (1µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Yang, Ho, Lau et al.: "Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages." in: International immunology, Vol. 17, Issue 11, pp. 1391-7, 2005 (PubMed).