Galanin Receptor 3 (GALR3) antibody

Antigen: Galanin Receptor 3 (GALR3)

Synonyms: Galnr3, GALR3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185603

Additional Information

Alternative name: Galanin Receptor 3

Gene ID: 8484

Swiss-Prot: NP_003605.1

Immunogen: Peptide with sequence C-QGPEPREGPVHGGE, from the C Terminus of the protein sequence according to NP_003605.1.

Cross-Reactivity: Human

Format: Liquid

Description: Principal Names: galanin receptor, family member 3, GALR3, OTTHUMP00000028820, galanin receptor 3. Official Symbol: GALR3

Specificity: Human

Molecular Weight: 39.6kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 37kDa band observed in Human Skin and Human Testes lysates (calculated MW of 39.6kDa according to NP_003605.1). Recommended concentration: 1-3µg/ml. IHC: In paraffin embedded Human Testis shows cellular membrane staining of spermatocytes. Recommended concentration, 4-6µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185603 (2µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Berger, Tuechler, Almer et al.: "Elevated expression of galanin receptors in childhood neuroblastic tumors." in: Neuroendocrinology, Vol. 75, Issue 2, pp. 130-8, 2002 (PubMed).

Swanson, Blackburn, Zhang et al.: "Anxiolytic- and antidepressant-like profiles of the galanin-3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 48, pp. 17489-94, 2005 (PubMed).