CDX2 antibody (Internal Region)

Catalog No. ABIN185604

The Goat Polyclonal anti-CDX2 antibody (ABIN185604) specifically detects CDX2 in IHC, WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CDX2 antibody (Internal Region) (ABIN185604)

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CDX2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CDX2 Antibody

Binding Specificity: Internal Region

Purpose: CDX2

Sequence: EQLSPGGQRR NLCE

Specificity: No cross-reactivity expected with CDX1 and 4

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EQLSPGGQRRNLCE, from the internal region of the protein sequence according to NP_001256.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Human Colon lysates (calculated MW of 33.5 kDa according to NP_001256.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Alternative Name: CDX2

Background: CDX2, caudal type homeo box transcription factor 2 , HGNC:1806, CDX-3, CDX3

Gene ID: 1045, 12591, 66019

NCBI Accession: NP_001256

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance