Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (HGS) antibody

Antigen: Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (HGS)

Synonyms: HRS, Vps27, ZFYVE8, Hrs, fi34f05, zgc:63492, wu:fi34f05, HGS, MGC134526, hgs, Hgr, HRS-2, l(2)23AB5, l(2)23Ad, l(2)23Ad(Hrs), vps27, DmelCG2903, CG2903, MGC68804

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185612

Additional Information

Alternative name: HRS / HGS

Gene ID: 9146, 15239 (mouse), 56084 (rat)

Immunogen: C-NRNYWEKKQEEARK

Format: Affinity-purified

Description: All names and symbols: HGS, hepatocyte growth factor-regulated tyrosine kinase substrate , HGNC:4897, HRS, ZFYVE8, human growth factor-regulated tyrosine kinase substrate. Official Symbol: HGS. RefSeq number(s): NP_004703.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 140kDa and 26kDa in Human lysates of monocytic leukemia cell line U937 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 86.2kDa according to NP_004703.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of Leydig cells. Recommended concentration, 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185612 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Kobayashi, Tanaka, Asao et al.: "Hrs, a mammalian master molecule in vesicular transport and protein sorting, suppresses the degradation of ESCRT proteins signal transducing adaptor molecule 1 and 2." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10468-77, 2005 (PubMed).