HGS antibody (Internal Region)

Catalog No. ABIN6262232

This Rabbit Polyclonal antibody specifically detects HGS in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HGS antibody (Internal Region) (ABIN6262232)

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-HGS Antibody

Binding Specificity: Internal Region

Specificity: HGS Antibody detects endogenous levels of total HGS.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HGS, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HGS

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Alternative Name: HGS

Background:

Description: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Gene: HGS

Molecular Weight: 86kDa

Gene ID: 9146

UniProt: O14964

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation