Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 2 (STAM2) antibody

Antigen: Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 2 (STAM2)

Synonyms: Hbp, STAM2A, STAM2B, DKFZp564C047, BB145015, 1200004O12Rik, 5730456G07Rik, stam2, MGC52950, STAM2, zgc:103628

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185613

Additional Information

Alternative name: STAM2

Gene ID: 10254, 56324 (mouse)

Immunogen: C-QSIDPTDSKPDSQD

Format: Affinity-purified

Description: All names and symbols: STAM2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 , HGNC:11358, DKFZp564C047, Hbp, STAM2A, STAM2B , STAM-like protein containing SH3 and ITAM domains 2, signal transducing adaptor molecule 2. Official Symbol: STAM2. RefSeq number(s): NP_005834.3

Specificity: No cross-reaction expected with STAM1. Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 70kDa band observed in Human Spleen lysates (calculated MW of 58.2kDa according to NP_005834.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pandey et al J Biol Chem. 2000 Dec 8,275(49):38633-9, PMID: 10993906). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Autophagy

Restrictions: For Research Use only

Images

ABIN185613 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kobayashi, Tanaka, Asao et al.: "Hrs, a mammalian master molecule in vesicular transport and protein sorting, suppresses the degradation of ESCRT proteins signal transducing adaptor molecule 1 and 2." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10468-77, 2005 (PubMed).