Tuberous Sclerosis 1 (TSC1) antibody

Antigen: Tuberous Sclerosis 1 (TSC1)

Synonyms: LAM, TSC, KIAA0243, MGC86987, hamartin, mKIAA0243, TSC1, MGC165718

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185619

Additional Information

Alternative name: Hamartin / TSC1 (isoform 1)

Gene ID: 7248, 64930 (mouse)

Immunogen: C-QLHIMDYNETHHEH

Format: Affinity-purified

Description: All names and symbols: TSC1, hamartin, tuberous sclerosis 1 , HGNC:12362, KIAA0243, LAM, MGC86987, TSC, tuberous sclerosis 1 protein, tumor suppressor. Official Symbol: TSC1. RefSeq number(s): NP_000359.1

Specificity: This antibody is expected to recognise isoform 1 (NP_000359.1) only. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22,9(12):1721-7, PMID: 10915759). Recommended concentration: 0.1-0.3µg/ml..

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185619 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Goncharova, Goncharov, Noonan et al.: "TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase." in: The Journal of cell biology, Vol. 167, Issue 6, pp. 1171-82, 2004 (PubMed).

Chan, Zhang, Roberts et al.: "Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas: biallelic inactivation of TSC1 or TSC2 leads to mTOR activation." in: Journal of neuropathology and experimental neurology, Vol. 63, Issue 12, pp. 1236-42, 2004 (PubMed).

Ballif, Roux, Gerber et al.: "Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 3, pp. 667-72, 2005 (PubMed).