Myosin IXB (MYO9B) antibody

Antigen: Myosin IXB (MYO9B)

Synonyms: MYR5, CELIAC4, MYO9B, fb53f01, wu:fb53f01, wu:fc47c07, si:dkeyp-66d1.8

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185642

Additional Information

Alternative name: Myosin IXB

Gene ID: 4650

Immunogen: C-KNDFEPVKQSKAQ

Description: All names and symbols: MYO9B , myosin IXB , CELIAC4, MYR5, myosin , celiac disease 4 . Official Symbol: MYO9B . RefSeq number(s): NP_004136.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 200kDa band observed in Human Liver lysates (calculated MW of 243kDa according to NP_004136.2). Recommended concentration: 2-6µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185642 (2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Monsuur, de Bakker, Alizadeh et al.: "Myosin IXB variant increases the risk of celiac disease and points toward a primary intestinal barrier defect." in: Nature genetics, Vol. 37, Issue 12, pp. 1341-4, 2005 (PubMed).