Fos-Related Antigen 1 (FOSL1) antibody

Antigen: Fos-Related Antigen 1 (FOSL1)

Synonyms: FRA, FRA1, fra-1, Fra1, AW538199, Fra-1, sb:eu506, si:ch211-145o7.4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185649

Additional Information

Alternative name: FRA1 / FOSL1

Gene ID: 8061, 14283 (mouse), 25445 (rat)

Immunogen: C-QREIEELQKQKER

Format: Affinity-purified

Description: All names and symbols: FOSL1, FRA1, FOS-like antigen 1 , HGNC:13718, fra-1 , FOS-like antigen-1. Official Symbol: FOSL1. RefSeq number(s): NP_005429.1

Specificity: No cross-reactivity is expected with V-FOS. Human, Mouse, Rat, Dog 271 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 26-28kDa band observed in Human Bone Marrow, Breast and Placenta lysates (calculated MW of 29.4kDa according to NP_005429.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185649 (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Belguise, Kersual, Galtier et al.: "FRA-1 expression level regulates proliferation and invasiveness of breast cancer cells." in: Oncogene, Vol. 24, Issue 8, pp. 1434-44, 2005 (PubMed).