Radixin (RDX) antibody
| Antigen |
|
| Synonyms |
DFNB24, AA516625, MGC95168, RDX |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (35), Rat (Rattus) (24), Mouse (Murine) (22), Dog (Canine) (6), Chicken (3), Cow (Bovine) (2), Guinea Pig (1), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1), Sheep (Ovine) (1)
|
|
Application
|
Immunofluorescence (IF) (22), Flow Cytometry (FACS) (19), Western Blotting (WB) (19), Enzyme Immunoassay (EIA) (10), Immunoprecipitation (IP) (5), ELISA (4), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN185665 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
Radixin
|
|
Gene ID
|
5962, 19684 (mouse), 315655 (rat)
|
|
Immunogen
|
C-QARDETKKTQND
|
|
Format
|
Affinity-purified
|
|
Description
|
All names and symbols: RDX, radixin. Official Symbol: RDX. RefSeq number(s): NP_002897.1
|
|
Specificity
|
Human, Mouse, Rat, Dog
|
|
Application Notes
|
Peptide ELISA: antibody detection limit dilution 1:4,000. Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Magendantz et al, J Biol Chem. 1995 Oct 27,270(43):25324-7., PMID: 7592691). Recommended concentration: 1-3µg/ml.
|
|
Purification
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Storage
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Research Area
|
Signaling, Cytoskeleton, Microfilaments
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Ivetic, Florey, Deka et al.: "Mutagenesis of the ezrin-radixin-moesin binding domain of L-selectin tail affects shedding, microvillar positioning, and leukocyte tethering." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33263-72, 2004 (PubMed).
|
Alternatives for antigen "Radixin (RDX)", type "Antibodies"
|
Hosts
|
Rabbit (23), Mouse (8), Goat (6)
|
|
Reactivities
|
Human (35), Rat (Rattus) (24), Mouse (Murine) (22), Dog (Canine) (6), Chicken (3), Cow (Bovine) (2), Guinea Pig (1), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1), Sheep (Ovine) (1)
|
|
Applications
|
Immunofluorescence (IF) (22), Flow Cytometry (FACS) (19), Western Blotting (WB) (19), Enzyme Immunoassay (EIA) (10), Immunoprecipitation (IP) (5), ELISA (4), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
Alternatives 
