Glutathione Peroxidase 7 (GPX7) antibody

Antigen: Glutathione Peroxidase 7 (GPX7)

Synonyms: GPX6, CL683, NPGPx, FLJ14777, AI327032, 3110050F08Rik, Ry2d1, MGC19204, olfactory, RP23-54E4.7, 1700020G18Rik, OBPII, GPX7, im:6902827, MGC147250, GLUTATHIONE PEROXIDASE 6, LSC803, PHGPX, T5C23.30, T5C23_30, F11C18.70, F11C18_70, glutathione peroxidase 7, gpx7

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185671

Additional Information

Alternative name: GPX7

Gene ID: 2882, 67305 (mouse), 298376 (rat)

Immunogen: CNQFGQQEPDSNK

Format: Affinity-purified

Description: All names and symbols: GPX7, glutathione peroxidase 7 [Homo sapiens] , HGNC:4559, CL683, FLJ14777, GPX6, NPGPx, non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase. Official Symbol: GPX7. RefSeq number(s): NP_056511.2

Specificity: Human

Synonyms: GPX6, CL683, NPGPx, FLJ14777, AI327032, 3110050F08Rik, Ry2d1, MGC19204, olfactory, RP23-54E4.7, 1700020G18Rik, OBPII, GPX7, im:6902827, MGC147250, GLUTATHIONE PEROXIDASE 6, LSC803, PHGPX, T5C23.30, T5C23_30, F11C18.70, F11C18_70, glutathione peroxidase 7, gpx7

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 20kDa band observed in Human Prostate lysates (calculated MW of 21.0kDa according to NP_056511.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185671 (0.3µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Utomo, Jiang, Furuta et al.: "Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43522-9, 2004 (PubMed).