Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 8 (SLC2A8) antibody

Antigen: Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 8 (SLC2A8)

Synonyms: GLUT8, GLUTX1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN185672

Additional Information

Alternative name: GLUT8 / SLC2A8

Gene ID: 29988

Immunogen: C-KGKTLEQITAH

Format: Affinity-purified

Description: All names and symbols: SLC2A8, solute carrier family 2, (facilitated glucose transporter) member 8 , HGNC:13812, GLUT8, GLUTX1 , glucose transporter X1. Official Symbol: SLC2A8. RefSeq number(s): NP_055395.2

Specificity: Human 477 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 80kDa and 35kDa in Human Peripheral Blood Mononucleocyte lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 50.8kDa according to NP_055395.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Added note: The observed 35kDa band corresponds to the calculated size of 34.4kDa of EAW87663.1 (314 aa long).

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Schiffer, Susztak, Ranalletta et al.: "Localization of the GLUT8 glucose transporter in murine kidney and regulation in vivo in nondiabetic and diabetic conditions." in: American journal of physiology. Renal physiology, Vol. 289, Issue 1, pp. F186-93, 2005 (PubMed).