GTF2IRD1 antibody (Internal Region)

Catalog No. ABIN185681

The Goat Polyclonal anti-GTF2IRD1 antibody is suitable to detect GTF2IRD1 in samples from Human. It has been validated for WB, ELISA, IF and FACS.

Quick Overview for GTF2IRD1 antibody (Internal Region) (ABIN185681)

Target: GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GTF2IRD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GTF2IRD1 Antibody

Binding Specificity: Internal Region

Purpose: GTF2IRD1

Sequence: NKFTKDTTKL EPAS

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKFTKDTTKLEPAS, from the internal region of the protein sequence according to NP_057412.1, NP_005676.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 106 kDa according to NP_057412.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GTF2IRD1

Target: GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))

Alternative Name: GTF2IRD1

Background: GTF2IRD1, GTF2I repeat domain containing 1 , HGNC:4661, CREAM1, GTF3, MUSTRD1, RBAP2, WBSCR11, WBSCR12, hMusTRD1alpha1 , GTF2I repeat domain-containing 1, Williams-Beuren syndrome chromosome region 11, general transcription factor 3, muscle TFII-I repeat

Gene ID: 9569, 57080, 246770

NCBI Accession: NP_057412, NP_005676