Fragile X Mental Retardation, Autosomal Homolog 1 (FXR1) antibody

Antigen: Fragile X Mental Retardation, Autosomal Homolog 1 (FXR1)

Synonyms: zgc:66226, wu:fb16f11, wu:fd18c10, Fxr1h, FXR1, MGC133793, Fxr1p, fxr1h, xfxr1, xFxr1p, DKFZp468H015

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185683

Additional Information

Alternative name: FXR1

Gene ID: 8087, 14359 (mouse), 361927 (rat)

Immunogen: C-RIEGDNENKLPRED

Format: Affinity-purified

Description: All names and symbols: FXR1, fragile X mental retardation, autosomal homolog 1 , fragile X mental retardation-related protein 1. Official Symbol: FXR1. RefSeq number(s): NP_005078.2, NP_001013456.1, NP_001013457.1

Specificity: This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1). Human, Mouse, Rat, Dog 621 aa, 539 aa, 536 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 80kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Khandjian et al Hum Mol Genet. 1998 Dec,7(13):2121-8., PMID: 9817930). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins

Restrictions: For Research Use only

Images

ABIN185683 (0.2µg/ml) staining of NIH/3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Coon, Matsunami, Stevens et al.: "Evidence for linkage on chromosome 3q25-27 in a large autism extended pedigree." in: Human heredity, Vol. 60, Issue 4, pp. 220-6, 2006 (PubMed).