Fibulin 5 (FBLN5) antibody
| Antigen |
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| Synonyms |
EVEC, UP50, ARMD3, DANCE, FIBL-5, FLJ90059, A55, zgc:103575, FBLN5, MGC128763, MGC108414, Fibulin-5, DKFZp469K0327 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (15), ELISA (10), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN185699 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Fibulin 5 / FBLN5
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Gene ID
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10516, 23876 (mouse), 29158 (rat)
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Immunogen
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C-RPIKGPREIQLDLE
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Format
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Affinity-purified
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Description
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All names and symbols: FBLN5, fibulin 5 , ARMD3, DANCE, EVEC, FLJ90059, UP50 , developmental arteries and neural crest epidermal growth factor-like, urine p50 protein. Official Symbol: FBLN5. RefSeq number(s): NP_006320.2
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Specificity
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Human, Mouse, Rat, Dog 448 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 50kDa band observed in Human Colon, Heart and Ovary lysates (calculated MW of 50.2kDa according to NP_006320.2). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Albig, Neil, Schiemann: "Fibulins 3 and 5 antagonize tumor angiogenesis in vivo." in: Cancer research, Vol. 66, Issue 5, pp. 2621-9, 2006 (PubMed).
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Alternatives for antigen "Fibulin 5 (FBLN5)", type "Antibodies"
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Hosts
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Rabbit (22), Mouse (8), Goat (3)
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Reactivities
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Human (28), Mouse (Murine) (22), Rat (Rattus) (22), Dog (Canine) (20), Pig (Porcine) (18), Cow (Bovine) (17), Chicken (16), Horse (Equine) (16), Rabbit (4)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (15), ELISA (10), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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