F-Box and Leucine-Rich Repeat Protein 3 (FBXL3) antibody

Antigen: F-Box and Leucine-Rich Repeat Protein 3 (FBXL3)

Synonyms: FBL3, FBL3A, FBXL3A, FBK, Ovtm, Fbl3a, Fbxl3a, AU041772, AW212966, fbxl3, MGC80572, FBXL3, MGC151742, F7A7.240, F7A7_240

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185700

Additional Information

Alternative name: FBXL3

Gene ID: 26224

Immunogen: KRGGRDSDRNSSEE-C

Format: Affinity-purified

Description: All names and symbols: FBXL3, F-box and leucine-rich repeat protein 3, FBL3, FBL3A, FBXL3A , F-box and leucine-rich repeat protein 3A, F-box protein Fbl3a. Official Symbol: FBXL3. RefSeq number(s): NP_036290.1

Specificity: Human, Dog 428 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 50-55kDa band observed in Human Adipose lysates (calculated MW of 48.7kDa according to NP_036290.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

ABIN185700 (1µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Albig, Neil, Schiemann: "Fibulins 3 and 5 antagonize tumor angiogenesis in vivo." in: Cancer research, Vol. 66, Issue 5, pp. 2621-9, 2006 (PubMed).