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Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) (internal) antibody
| Antigen | Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) |
| Synonyms | CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1 |
| Epitope |
 Alternatives internal |
| Clonality | Polyclonal |
| Host |
Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN185703 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | CARD15 / NOD2 (Internal) |
| Gene ID | 64127, 257632 (mouse), 291912 (rat) |
| Immunogen | KFRFTDRERHCSPTD |
| Format | Affinity-purified |
| Description | All names and symbols: CARD15, NOD2, caspase recruitment domain family, member 15, ACUG, BLAU, CD, IBD1, NOD2B, PSORAS1, LRR-containing protein, NOD2 protein, caspase recruitment domain protein 15, inflammatory bowel disease protein 1, nucleotide-binding oligomerization domain 2,. Official Symbol: CARD15. RefSeq number(s): NP_071445.1 |
| Specificity | Human, Mouse, Rat 1040 aa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 100kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN185703 (0.1µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
| Publications |
Keshav: "Paneth cells: leukocyte-like mediators of innate immunity in the intestine." in: Journal of leukocyte biology, Vol. 80, Issue 3, pp. 500-8, 2006 (PubMed).
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Alternatives
Alternatives for antigen "Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2)", type "Antibodies"
| Hosts | Rabbit (32), Goat (3), Mouse (2), Rat (1) |
| Reactivities | Human (34), Mouse (Murine) (14), Rat (Rattus) (10), Dog (Canine) (4), Pig (Porcine) (3), Cow (Bovine) (2), Monkey (2), Rabbit (2), Chicken (1), Zebrafish (Brachydanio rerio) (1) |
| Applications | Immunofluorescence (IF) (20), Western Blotting (WB) (20), Flow Cytometry (FACS) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Enzyme Immunoassay (EIA) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), ELISA (1), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | C-Term (3), AA 125-140 (1), N-Term (1), internal (1) |
