Serologically Defined Breast Cancer Antigen NY-BR-24 (SEPT1) antibody

Antigen: Serologically Defined Breast Cancer Antigen NY-BR-24 (SEPT1)

Synonyms: LARP, SEP1, DIFF6, PNUTL3, MGC20394

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN185707

Additional Information

Alternative name: Septin 1 / SEPT1

Gene ID: 1731, 54204 (mouse), 293507 (rat)

Immunogen: C-GARDRASRSKLSRQ

Format: Affinity-purified

Description: All names and symbols: SEPT1, septin 1 , DIFF6, LARP, MGC20394, PNUTL3, SEP1 , differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase), serologically defined breast cancer antigen NY-BR-24. Official Symbol: 01.09.01. RefSeq number(s): NP_443070.1

Specificity: Human, Mouse, Rat, Dog 367 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 30kDa band in Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 42.0 kDa according to mouse NP_059489.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Qi, Yu, Liu et al.: "Septin1, a new interaction partner for human serine/threonine kinase aurora-B." in: Biochemical and biophysical research communications, Vol. 336, Issue 3, pp. 994-1000, 2005 (PubMed).