Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP) (internal) antibody

Antigen: Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP)

Synonyms: FLAP, Flap, fj65d02, zgc:63982, wu:fj65d02, ALOX5AP, flap, MGC108020, MGC140744, Alox5ap, al5ap

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185717

Additional Information

Alternative name: FLAP / ALOX5AP (internal)

Gene ID: 241

Immunogen: C-HKVEHESRTQNGR

Format: Affinity-purified

Description: All names and symbols: ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, five-lipoxygenase activating protein. Official Symbol: ALOX5AP. RefSeq number(s): NP_001620.2

Specificity: Human, Dog 161 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185717 (0.1µg/ml) staining of Human U937 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kaaman, Ryden, Axelsson et al.: "ALOX5AP expression, but not gene haplotypes, is associated with obesity and insulin resistance." in: International journal of obesity (2005), Vol. 30, Issue 3, pp. 447-52, 2006 (PubMed).