CILP antibody (AA 603-846)

Catalog No. ABIN1858408

Product Details anti-CILP Antibody

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Binding Specificity: AA 603-846

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CILP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Cartilage Intermediate Layer Protein (CILP)

Specificity: The antibody is a rabbit polyclonal antibody raised against CILP. It has been selected for its ability to recognize CILP in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cartilage Intermediate Layer Protein (CILP) corresdonding to Ile603~Ala846 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.23 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CILP

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Alternative Name: Cartilage Intermediate Layer Protein (CILP Products)

Background: Nucleotide Pyrophosphohydrolase, Cartilage intermediate-layer protein