CILP antibody

Catalog No. ABIN7227908

The Rabbit Polyclonal anti-CILP antibody (ABIN7227908) specifically detects CILP in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for CILP antibody (ABIN7227908)

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CILP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CILP Antibody

Purpose: Rabbit Anti-CILP1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CILP1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CILP1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CILP

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Alternative Name: CILP1

Background: Cartilage intermediate layer protein 1, CILP-1, Cartilage intermediate-layer proteinMajor alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. CILP encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins, an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease.

Molecular Weight: 130kD

Gene ID: 8483

UniProt: O75339