AGRN antibody (AA 1856-2045)

Catalog No. ABIN1866584

The Rabbit Polyclonal anti-AGRN antibody has been validated for IHC, WB, IP and ICC. It is suitable to detect AGRN in samples from Human.

Quick Overview for AGRN antibody (AA 1856-2045) (ABIN1866584)

Target: AGRN (Agrin (AGRN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGRN antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-AGRN Antibody

Binding Specificity: AA 1856-2045

Purpose: Polyclonal Antibody to Agrin (AGRN)

Specificity: The antibody is a rabbit polyclonal antibody raised against AGRN. It has been selected for its ability to recognize AGRN in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Agrin (AGRN) corresdonding to Leu1856~Pro2045 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: AGRN

Background: Agrin Proteoglycan

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development