AGRN antibody (AA 1900-1980)

Catalog No. ABIN7227240

The Rabbit Polyclonal anti-AGRN antibody is suitable to detect AGRN in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for AGRN antibody (AA 1900-1980) (ABIN7227240)

Target: AGRN (Agrin (AGRN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGRN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AGRN Antibody

Binding Specificity: AA 1900-1980

Purpose: Rabbit Anti-AGRIN Polyclonal Antibody

Specificity: The antibody detects endogenous levels of AGRIN protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human AGRIN protein at AA range: 1900-1980

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: AGRIN

Background: Agrin, Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, C90, Agrin C-terminal 22 kDa fragment, C22AGRN encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in AGRN was found. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 224kD

Gene ID: 375790

UniProt: O00468

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development