AGRN antibody (AA 968-1130)

Catalog No. ABIN7143601

Product Details anti-AGRN Antibody

Target: AGRN (Agrin (AGRN))

Binding Specificity: AA 968-1130

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGRN antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Agrin protein (968-1130AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: AGRN (AGRN Products)

Synonyms: AGRIN antibody, agrn antibody, wu:fb57e07 antibody, Agrin antibody, nmf380 antibody, AGR antibody, HSPG antibody, agrin antibody, LOC465392 antibody, agrn antibody, AGRN antibody, Agrn antibody

Background:

Background: Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This secreted isoform forms a bridge, after release from motor neurons, to basal lamina through binding laminin via the NtA domain.

Aliases: AGRIN antibody, Agrin C-terminal 22 kDa fragment antibody, Agrin proteoglycan antibody, AGRIN_HUMAN antibody, Agrn antibody, C22 antibody, C90 antibody, FLJ45064 antibody, OTTHUMP00000044043 antibody

UniProt: O00468

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development