FASL antibody

Catalog No. ABIN187288

This Rat Monoclonal antibody specifically detects FASL in IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for FASL antibody (ABIN187288)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A11

Product Details anti-FASL Antibody

Specificity: Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced apoptosis of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and some tumor cells. Species Reactivity: Mouse. Others not tested.

Immunogen: Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein

Isotype: IgM

Application Details

Application Notes: Flow cytometry Immunohistochemistry: Excellent for this application. Frozen Sections- Use 20 µg/mL, incubate 1 hr at room temp. Method: Cryostat sections (6-8 µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at RT with 20 µg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Paraffin-Embedded Sections- Dewax in xylene (2 x 10 min), EtOH 100% (2 x 10 min), block endogenous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H2O, unmask epitopes by heating slides in citrate buffer (0.01 M, pH 6.0) 2 x 5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC). The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µL of 1 mg/mL monoclonal antibody in PBS with 0.02% sodium azide. Purity 95% by SDS-PAGE.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 / Fas Ligand

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity