TACI antibody

Catalog No. ABIN187592

The Rat Monoclonal anti-TACI antibody is suitable to detect TACI in samples from Mouse. It has been validated for FACS, IHC (fro) and Func.

Quick Overview for TACI antibody (ABIN187592)

Target: TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TACI antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 8F10

Product Details anti-TACI Antibody

Specificity: Recognizes mouse TACI. Species Reactivity: Mouse. Other species not tested.

Immunogen: Rat RBL cells expressing mouse TACI.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry Immunohistochemistry: Frozen sections Functional application: Inhibition The researcher should determine the optimal dilution for a specific application. Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse TACI (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 µg/mL of rat anti-TACI antibody (MC-575). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

Restrictions: For Research Use only

Handling

Buffer: 100 µg antibody purified from concentrated hybridoma tissue culture supernatant at 1 mg/mL in PBS. Purity 95% as determined by SDS-PAGE.

Storage: 4 °C

Target Details for TACI

Target: TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))

Alternative Name: CD267 / TACI