ASK1 antibody

Catalog No. ABIN187833

Product Details anti-ASK1 Antibody

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects ASK1. Species Reactivity: Human, other species not tested.

Immunogen: Synthetic peptide corresponding to amino acids 1356 to 1375 of human ASK1 protein.

Application Details

Application Notes: Western blot: Use 0.5-1 µg/ml. Detects a 155 kDa band in SW1353 whole cell lysate. The optimal dilution for a specific application should be determined by the researcher. Note: immunogen sequence differs in mouse by last two amino acids.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Provided at 0.5 mg/ml in PBS solution containing 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: MEKK5 / ASK1 (MAP3K5 Products)

Synonyms: ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody

Background: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibit TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response