Fibronectin antibody

Catalog No. ABIN3071578

The Mouse Monoclonal anti-Fibronectin antibody (Clone FN30-8) (ABIN3071578) specifically detects Fibronectin in WB, ELISA and InhA. The antibody is reactive with Human samples.

Quick Overview for Fibronectin antibody (ABIN3071578)

Target: Fibronectin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Inhibition Assay (InhA)

Clone: FN30-8

Product Details anti-Fibronectin Antibody

Purpose: Anti-Human Fibronectin Mouse Monoclonal Antibody

Specificity: This antibody recognizes an epitope in the cell-binding domain of human fibronectin and can inhibit cell adhesion.

Cross-Reactivity: Human

Immunogen: Human plasma fibronectin

Isotype: IgG1

Application Details

Application Notes: ELISA: 1-10 μg/mL with immobilized antigen
Western Blot: 1-10 μg/mL, reducing or non-reducing conditions
Cell Adhesion Inhibition Assay: 0.1-1.0 μg/mL on 10 μg/mL fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells.
Immunohistochemistry: 1-10 μg/mL, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.
These are recommended concentrations.
End users should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute lyophilized antibody in 200 µL of distilled water (final concentration 2.0 mg/mL).

Buffer: PBS, pH 7.4, 1 % BSA, lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectin,Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}., [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.,Fibronectin is a 440 kDa glycoprotein of the extracellular matrix that binds to integrins, collagen, fibrin, and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis.,Secreted, extracellular space, extracellular matrix,FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3]

UniProt: P02751