Rpa12p (RPA12) antibody

Catalog No. ABIN190724

Product Details

Target: Rpa12p (RPA12)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 13.6
This product has previously been labelled as anti-At3g25940 transcription factorS-II (TFIIS) domain-containing protein.

Purification: serum

Immunogen: KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.

Application Details

Application Notes: 1 : 2 000 with standard ECL (WB)

Comment:

This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 143 μL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: Rpa12p (RPA12)

Alternative Name: A12.2

Synonyms: RRN4 antibody, DNA-directed RNA polymerase I subunit RPA12 antibody, RPA12 antibody

Background: AGI Code: At3g25940
RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.

Molecular Weight: 13.6

UniProt: Q9LU97