23 Kda Protein of The Oxygen Evolving Complex (Oec) of Psii (Psbp) antibody

Antigen: 23 Kda Protein of The Oxygen Evolving Complex (Oec) of Psii (Psbp)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Barley (Hordeum vulgare), Jack Pine (Pinus banksiana), Spinach (Spinacia oleracea), Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB)

Catalog no.: ABIN190744

Additional Information

Immunogen: native, purified 23 kDa protein from Spinacia oleracea

Format: Lyophilized

Description: PSII reaction centre components are generating the redox potential required todrive highly oxidizing water splitting reaction. Four Mn atoms are present on alumenal surface and form the catalyctic site of the water-splitting reactionwhich is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa(PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsicprotein is also termed the Mn-stabilizing protein (MSP), however recent evidencesshown that it is C-terminal domain of PsbA (D1) protein which is involved in in theassembly and stabilization of the OEC.

Specificity: Predicted reactivity: dicots including: Pisum sativum, Solanum lycopersicum, monocots including:Oryza sativa, trees including: Populus balsamifera, Pinus monticola. Not reactive in: Synechococcus sp. PCC 7942.

Application Details

Application Notes: Recommended Dilution 1:2000 - 1: 5000 with standard ECL (WB).

Buffer: total IgG in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Arabidopsis thaliana, Horderum vulgare, Chlamydmononas reinhardtii, Synechococcus sp. 7942 (2 ug of total cellular protein was loaded per lane)

Publications

Publications: Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, 2011 (PubMed).