Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody

Details for Product No. ABIN190755
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Antigen
Synonyms p40phox, AI451400, NCF, P40PHOX, SH3PXD4
Epitope
Internal Region
(17), (14), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(80), (43), (33), (30), (24), (14), (12), (2)
Host
Goat
(68), (9), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(48), (29), (20), (17), (13), (3), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN190755
374.27 $
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Immunogen Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.
Specificity This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NCF4 / P40PHOX
Background Synonyms: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa, CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)
Gene ID 4689
NCBI Accession NP_038202, NP_000622
Research Area Autophagy
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody ABIN190755 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Rioux, Xavier, Taylor et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).

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