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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody

Details for Product No. ABIN190755, Supplier: Log in to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
Epitope
Internal Region
17
10
10
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
105
47
37
7
5
3
3
1
1
Host
Goat
79
18
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
65
38
28
22
15
4
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.
Blocking Peptide Blocking peptide for this product available: ABIN369246
Specificity This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NCF4 / P40PHOX (NCF4 Antibody Abstract)
Background Synonyms: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa, CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)
Gene ID 4689
NCBI Accession NP_038202, NP_000622
Research Area Autophagy
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody (ABIN190755) ABIN190755 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Pr...
Background publications Rioux, Xavier, Taylor et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. ..." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).