NCF4 antibody (C-Term)

Catalog No. ABIN391905

The Rabbit Polyclonal anti-NCF4 antibody (Clone RB18508) (ABIN391905) specifically detects NCF4 in WB, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for NCF4 antibody (C-Term) (ABIN391905)

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB18508

Product Details anti-NCF4 Antibody

Binding Specificity: AA 260-289, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This NCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human NCF4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: NCF4

Background: NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2(NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity.

Molecular Weight: 39032

Gene ID: 4689

NCBI Accession: NP_000622, NP_038202

UniProt: Q15080