NCF4 antibody

Catalog No. ABIN7268900

The Rabbit Monoclonal anti-NCF4 antibody is suitable to detect NCF4 in samples from Human. It has been validated for WB, IHC and IP.

Quick Overview for NCF4 antibody (ABIN7268900)

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-NCF4 Antibody

Purpose: NCF4/p40-phox Rabbit mAb

Cross-Reactivity: Human, Mouse

Characteristics: Monoclonal Antibodies

Purification: Affinity purification

Immunogen: A synthesized peptide derived from human NCF4/p40-phox.

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: NCF4

Background: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,NCF4, CGD3, NCF, P40PHOX, SH3PXD4, neutrophil cytosol factor 4,Cancer,Signal Transduction,Cell Biology & Developmental Biology,Endocrine & Metabolism,Mitochondrial metabolism,Mitochondrial markers,NCF4

Molecular Weight: 39kDa

Gene ID: 4689

UniProt: Q15080