Pancreas Specific Transcription Factor, 1a (PTF1A) antibody

Antigen: Pancreas Specific Transcription Factor, 1a (PTF1A)

Synonyms: bHLHa29, PTF1-p48, PTF1p48, PTF1, MGC112216, zgc:112216, si:zc142h2.2, PTF1A, XPtf1a, Ptf1a/p48

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190768

Additional Information

Alternative name: PTF1A / PFT1-P48

Gene ID: 256297, 19213 (mouse), 117034 (rat)

Immunogen: C-WTDEKQLKEQN

Format: Affinity-purified

Description: All names and symbols: PTF1A, PTF1-p48, pancreas specific transcription factor, 1a, bHLH transcription factor p48, class II bHLH protein PTF1A, exocrine pancreas-specific transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1, pancreas transcription factor 1 subunit alpha. Official Symbol: PTF1A. RefSeq number(s): NP_835455.1

Specificity: Human, Mouse, Rat, Dog, Zebrafish

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 35.0kDa according to NP_835455.1. Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190768 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Pascual, Abasolo, Mingorance-Le Meur et al.: "Cerebellar GABAergic progenitors adopt an external granule cell-like phenotype in the absence of Ptf1a transcription factor expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 12, pp. 5193-8, 2007 (PubMed).