ROBO3 antibody (Internal Region)

Catalog No. ABIN190792

This Goat Polyclonal antibody specifically detects ROBO3 in ELISA. It exhibits reactivity toward Mouse.

Quick Overview for ROBO3 antibody (Internal Region) (ABIN190792)

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ROBO3 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-ROBO3 Antibody

Binding Specificity: Internal Region

Purpose: Robo3 (mouse)

Sequence: RRQKQRKELS HYT

Specificity: Both variants represent identical protein

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RRQKQRKELSHYT, from the internal region of the protein sequence according to NP_035378.1, AAD11628.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 75-80 kDa band in Mouse Brain and Rat Brain lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcul

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: Robo3

Background: Robo3, roundabout homolog 3 (Drosophila), Rbig1, RIG1, Rig-1, Robo3a, Robo3b, retinoblastoma inhibiting gene 1

Gene ID: 19649, 315564

NCBI Accession: NP_035378