Roundabout Homolog 3 (Drosophila) (ROBO3) antibody

Antigen: Roundabout Homolog 3 (Drosophila) (ROBO3)

Synonyms: CG14345, CT17158, Robo-3, Robo3, dRobo-3, DmelCG5423, CG5423, ROBO3, robo3, DKFZp468G0223

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190792

Additional Information

Alternative name: Robo3 (mouse)

Gene ID: 19649 (mouse), 315564 (rat)

Immunogen: C-RRQKQRKELSHYT

Format: Affinity-purified

Description: All names and symbols: Robo3, roundabout homolog 3 (Drosophila), Rbig1, RIG1, Rig-1, Robo3a, Robo3b, retinoblastoma inhibiting gene 1. Official Symbol: Robo3. RefSeq number(s): NP_035378.1, AAD11628.1

Specificity: Both variants represent identical protein Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).