ClpB Caseinolytic Peptidase B Homolog (E. Coli) (CLPB) antibody

Antigen: ClpB Caseinolytic Peptidase B Homolog (E. Coli) (CLPB)

Synonyms: Skd3, AL118244, SKD3, HSP78, FLJ13152

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190796

Additional Information

Alternative name: CLPB

Gene ID: 81570

Immunogen: C-DKDSKTRRLDIRAP

Format: Affinity-purified

Description: All names and symbols: CLPB, ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of potassium transport defect 3. Official Symbol: CLPB. RefSeq number(s): NP_110440.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Publications

Publications: Satpute-Krishnan, Langseth, Serio: "Hsp104-dependent remodeling of prion complexes mediates protein-only inheritance." in: PLoS biology, Vol. 5, Issue 2, pp. e24, 2007 (PubMed).