Chloride Intracellular Channel 4 (CLIC4) antibody

Antigen: Chloride Intracellular Channel 4 (CLIC4)

Synonyms: H1, huH1, p64H1, CLIC4L, MTCLIC, FLJ38640, DKFZp566G223, cb142, wu:fb33c05, wu:fc30h10, clic4, MGC89006, MGC79614, CLIC4, DKFZp459A0325

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN190810

Additional Information

Alternative name: CLIC4

Gene ID: 25932, 29876 (mouse), 83718 (rat)

Immunogen: NGLKEEDKEPLIE-C

Format: Affinity-purified

Description: All names and symbols: CLIC4, chloride intracellular channel 4, CLIC4L, DKFZP566G223, FLJ38640, H1, huH1, p64H1 , chloride intracellular channel 4 like. Official Symbol: CLIC4. RefSeq number(s): NP_039234.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Approx 30kDa band observed in human kidney and human placenta lysates (calculated MW of 28.8kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar,276(3 Pt 2):F398-408., PMID: 10070163). Recommended concentration: 0.1-0.3µg/ml. Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5µg/ml with overnight incubation.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism, Cell Cycle, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

ABIN190810 (0.1µg/ml) staining of human kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN190810 (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.

Publications

Publications: Suh, Crutchley, Koochek et al.: "Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 1, pp. 121-31, 2007 (PubMed).