Acyl-CoA Oxidase 2, Branched Chain (Acox2) antibody

Antigen: Acyl-CoA Oxidase 2, Branched Chain (Acox2)

Synonyms: BCOX, BRCOX, THCCox, BRCACOX, MGC83074, ACOX2, MGC159638, THCA, DKFZp469P0525

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190845

Additional Information

Alternative name: ACOX2

Gene ID: 8309, 93732 (mouse), 252898 (rat)

Immunogen: C-HQSRLRPSDPEAK

Format: Affinity-purified

Description: All names and symbols: ACOX2, acyl-Coenzyme A oxidase 2, branched chain, BCOX, BRCACOX, BRCOX, THCCox, Peroxisomal branched chain acyl-CoA oxidase, THCA-CoA oxidase, Trihydroxycoprostanoyl-CoA oxidase. Official Symbol: ACOX2. RefSeq number(s): NP_003491.1

Specificity: Please note this antibody was designed using the mouse sequence, which differs by 1 amino acids from the human sequence. Human, Mouse, Rat, Cow, Rabbit

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Fatty Acids, Metabolism, Organelles

Restrictions: For Research Use only

Images

ABIN190845 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Baumgart, Vanhooren, Fransen et al.: "Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13748-53, 1997 (PubMed).