Acyl-CoA Oxidase 2, Branched Chain (Acox2) antibody

Antigen: Acyl-CoA Oxidase 2, Branched Chain (Acox2)

Synonyms: BCOX, BRCOX, THCCox, BRCACOX, MGC83074, ACOX2, MGC159638, THCA, DKFZp469P0525

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190846

Additional Information

Alternative name: ACOX2 (Human)

Gene ID: 8309

Immunogen: C-QWAQKSPTNTQENP

Format: Affinity-purified

Description: All names and symbols: ACOX2, acyl-Coenzyme A oxidase 2, branched chain , BCOX, BRCACOX, BRCOX, THCCox , Peroxisomal branched chain acyl-CoA oxidase, THCA-CoA oxidase, Trihydroxycoprostanoyl-CoA oxidase. Official Symbol: ACOX2. RefSeq number(s): NP_003491.1

Specificity: Human, Rabbit

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 75kDa band observed in Human Liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190846 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Baumgart, Vanhooren, Fransen et al.: "Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13748-53, 1997 (PubMed).