Actin-Like 7b (Actl7b) antibody

Antigen: Actin-Like 7b (Actl7b)

Synonyms: Tact1, MGC151192, MGC151194, ACTL7B

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190854

Additional Information

Alternative name: Actin-like 7B (mouse)

Gene ID: 11471 (mouse), 313183 (rat)

Immunogen: C-DELHVDYELPDGK

Format: Affinity-purified

Description: All names and symbols: actin-like 7B, ACTL7B, MGC151192, MGC151194, Tact1, actin-like 7-beta, t-actin 1. Official Symbol: Actl7b. RefSeq number(s): NP_079547.1

Specificity: This antibody is not expected to cross-react with Actin-like 7A. Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in Rat Testis lysates (calculated MW of 45.5kDa according to Rat NP_001020588.1 and 45.6kDa according to Mouse NP_079547.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190854 (0.5µg/ml) staining of Rat Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Chadwick, Mull, Helbling et al.: "Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31." in: Genomics, Vol. 58, Issue 3, pp. 302-9, 1999 (PubMed).