Chromosome 14 Open Reading Frame 68 (C14orf68) antibody

Antigen: Chromosome 14 Open Reading Frame 68 (C14orf68)

Synonyms: HDMCP, HMFN1655

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN190872

Additional Information

Alternative name: C14orf68

Gene ID: 283600

Immunogen: C-RYGNPDAKPTKAD

Format: Affinity-purified

Description: All names and symbols: C14orf68, chromosome 14 open reading frame 68 , HDMCP, HMFN1655 , hepatocellular carcinoma-downregulated mitochondrial carrier protein. Official Symbol: C14orf68. RefSeq number(s): NP_997000.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Organelles

Restrictions: For Research Use only

Images

ABIN190872 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Tan, Ooi, Aw et al.: "Cloning and identification of hepatocellular carcinoma down-regulated mitochondrial carrier protein, a novel liver-specific uncoupling protein." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 45235-44, 2004 (PubMed).