Ubiquitin Associated and SH3 Domain Containing, A (UBASH3A) (internal) antibody

Antigen: Ubiquitin Associated and SH3 Domain Containing, A (UBASH3A)

Synonyms: TULA, CLIP4, STS-2, Sts-2, C330001M22, 5830413C03Rik, UBASH3A

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN190877

Additional Information

Alternative name: STS2 / TULA (internal)

Gene ID: 53347

Immunogen: QQCSTPDGKYYRPD

Format: Affinity-purified

Description: All names and symbols: TULA , STS2 CBL-interacting protein 4, UBASH3A, ubiquitin associated and SH3 domain containing, A , T-cell ubiquitin ligand protein, Suppressor of T-cell receptor signaling 2, CLIP4. Official Symbol: UBASH3A. RefSeq number(s): NP_061834.1, NP_001001895.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1). Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration, 2.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190877 (2.5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Kowanetz, Crosetto, Haglund et al.: "Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32786-95, 2004 (PubMed).